Ø In vitro drug efficacy testing：Cell proliferation, antibacterial, antiviral, binding with targets, exploration of mechanism of action, etc

Ø In vivo efficacy evaluation：Covering a variety of animal models such as infectious diseases, tumors, neurological/respiratory/cardiovascular/urinary/digestive diseases, and metabolic related diseases

一、 Antitumor class

Ø In vitro screening of anti-tumor drugs

l Cell proliferation and toxicity testing

l Cell cloning test

l Cell migration test

l Angiogenesis test

l PDO

Ø In vivo anti-tumor test

l CDX model：Subcutaneous transplantation, in-situ implantation (allogeneic, xenogeneic)

l PDX model：PATIENT DERIVED TUMOR Xenograft (PDX): The human tumor xenotransplantation model is established by directly transplanting the patient's tumor tissue to the immune deficient mice. The human tumor xenotransplantation model retains the characteristics of the parent tumor in histopathology, molecular biology and gene levels, and has good clinical efficacy predictability. Therefore, the platform is currently widely used in the development of new drugs, especially in the clinical trials of target drugs, patient screening, and the study of predictive biomarkers.

l PBMC、Humanization model of hematopoietic stem cells：Apply immunodeficient animals for humanization construction, and based on this, conduct research on the anti-tumor efficacy of the test substance.

  Solution example: Research ideas and solutions for the efficacy of a certain BITE anti MM drug

1. Cellular

1.1 Detection of tumor cell killing effect (2D culture)

1.2 T cell infiltration (3D microspheres)

2. Animal level

2.1 PBMC humanized mouse combined with subcutaneous transplantation tumor

2.2 PBMC humanized mouse combined with in situ tumor transplantation

2.3 huCD34+HSC mouse combined with orthotopic tumor transplantation

二、 Anti Neuropsychiatric

Taichu Pharmacodynamics Department has established a number of evaluation technologies for mental system disease models, including antidepressants, anti Alzheimer's drugs, sedatives, hypnotics, anti anxiety drugs, analgesics, anticonvulsants, anti Parkinson's drugs, and anti schizophrenia drugs, which can comprehensively evaluate a class of innovative drugs at the molecular level, cellular level, ex vivo, in vivo, and other levels.

The advanced CognitionWall Discrimination learning memory function tracking test system from the Ministry of Pharmacology, with 24-hour continuous tracking testing, can effectively identify memory function changes in dual transgenic mice with Alzheimer's disease that are still in the early stage of the disease, and eliminate the disadvantages of Morris water maze stress interference and short-term testing.

Ø Anti Alzheimer's drugs

Ø Anti Parkinson's drugs

Ø antiepileptic drugs

Ø Anti spinal cord/head injury drugs

Ø Anti cerebral vasospasm drugs

Ø analgesics

Ø Antidepressants

Solution example: Research ideas and solutions for the efficacy of anti spinal cord injury drugs

1. Determine corresponding indications (spinal cord injury) based on the specific effects of the test substance

2. Select appropriate in vitro and in vivo models for efficacy evaluation (heavy object hitting T10 spinal cord)

3. The experimental design includes the study of conventional dose-effect relationship and time effect relationship

4. Determine detection indicators:

4.1 Behavioral studies;

4.2 Electrophysiology: Detection of integrity of neural impulse transmission

4.3 Pathology: Neurofibrodegeneration, nerve regeneration, etc

5. Finally, the pharmacological characteristics of the test substance were determined